Publications

201 papers, 1984–2026.

Our group is dedicated to advancing the field of chemical biology and drug discovery.

2026

Bendre SV, Wang Y, Hajyousif B, K C R, Bhogale SG, Pradeep D, Krawczynska N, Schane CP, Weisser E, Singh A, Han S, Kim H, Kockaya L, Das Gupta A, Nelczyk AT, Vidana Gamage HE, Fei Y, Rodríguez-Casiano D, Guo X, Li H, Deaton RJ, Mo F, Sverdlov M, Gann PH, Sinha S, Sahni S, Wang K, Van Bortle K, Tajkorshid E, Woodward WA, Cho W, Nelson ER. Cholesterol efflux protein, ABCA1, supports anti-cancer functions of myeloid immune cells Sci Adv. 2026 Jan 2;12(1):eadx5490. doi: 10.1126/sciadv.adx5490. Epub 2026 Jan 1. PMID: 41477845
PubMed 41477845 DOI
Cho, W. Boosting immunotherapy by restoring cryptic APC function Cell Res. 2026. https://doi.org/10.1038/s41422-026-01219-7
PubMed 41540204 DOI
Kavrakova, S., Sharma, A., Ristevska, E., Guo, X., Zalejski, J., Cho, W. (2026) “Site-specific cholesterol depletion therapy for gastric cancer” J. Lipid Res. 101125.
PubMed 42612977 DOI
Loughran, R.M., Arora, G.K., Sun, J., Llorente, A., Crabtree, S., Zhang, C.Y., Ly, K., Huynh, R.L., Cho, W., Emerling, B.M. (2026) “Noncanonical PI(4,5)P2 coordinates lysosome positioning through cholesterol trafficking” Sci. Adv. 12, eaeb8658.
PubMed 42172317 DOI
Sharma, A., Cho, W. (2026) “Pharmacological modulation of lipid signaling” Methods Enzymol. 728, 369-387.
PubMed 41895918 DOI

2025

Hajirahimkhan A, Bartom ET, Chung CH, Guo X, Berkley K, Hosseinibarkooie S, Assadi Z, Weng SHS, Moellering R, Lee O, Chen R, Cho W, Chandrasekaran S, Clare SE, Khan SA. Reprogramming SREBP1-dependent lipogenesis and inflammation in high-risk breast with licochalcone A: A novel path to cancer prevention. Int J Cancer. 2025 Nov 27. doi: 10.1002/ijc.70226. Online ahead of print. PMID: 41310893
PubMed 41310893 DOI
Kim DH, Hong MT, Bocharov EV, Park S, Bershatsky YV, Volynsky PE, Kim E, Efremov RG, Cho W, Park SY, Ryu SH. EGFR activation requires cholesterol interaction at the inner leaflet of the plasma membrane. Sci Adv. 2025 Dec 19;11(51):eadx2398. doi: 10.1126/sciadv.adx2398. Epub 2025 Dec 19. PMID: 41417903
PubMed 41417903 DOI
Sejeong Shin, Min-Joon Han, Ishika Patel, Alia M. Welsh, Maria Sverdlov, Wonhwa Cho, Stephanie Cologna, Philippe P. Roux, Sang-Oh Yoon, mTOR inhibition reprograms cellular lipid homeostasis by inducing alternative lipid uptake and promoting cholesterol transport, Molecular Cell. 2025 Sep 18;85(18):3486-3504.e7. PMID: 40972529
PubMed 40972529 DOI
Sharma, A., Zalejski, J., Bendre, SV, Simona Kavrokova, S., Hasdemir, HS, Defne Gorgun Ozgulbas, DG, Sun, J, Pathmasiri, KC, Shi, R., Aloulou A., Berkley, K, Delisle, CF, Young Wang, Y., Weisser, E, Buweneka, P, Pierre-Jacques, D, Mukherjee, S, Abbasi, DA, Lee, D, Wang, B, Gevorgyan, V, Cologna, SM, Tajkhorshid, E., Nelson, ER, and Cho, W. Cholesterol-targeting Wnt/β-catenin signaling inhibitors for colorectal cancer, Nature Chemical Biology, 2025, Sep; 21(9): 1376-1386 PMID: 40240631. PMCID: PMC12353659. [Featured in Nature Chemical Biology News & Views and Trends in Pharmacological Sciences Spotlight]
PubMed 40240631 DOI

2023

Cho W, Berkley K, Sharma A. Lipid Binding of SH2 Domains. Methods Mol Biol. 2023;2705:239-253. doi: 10.1007/978-1-0716-3393-9_13.PMID: 37668978
PubMed 37668978 DOI
Jiachen Sun, Seohyeon Song, Indira Singaram, Yusi Hu, (Jean Zhao) Volker Haucke, and Wonhwa Cho. PI(3,5)P2 Controls the Signaling Activity of Class I PI3K: Its Implication in Cancer. bioRxiv. doi: 10.1101/2023.01.25.525550.
PubMed 36747849 DOI
Ning D, Wang ZG, Wang L, Tian YF, Jing F, Jiang LH, Zhang MQ, Liu YY, Pang DW, Cho W, Liu SL., Lipid-Centric Design of Plasma Membrane-Mimicking Nanocarriers for Targeted Chemotherapeutic Delivery. Advanced Materials. 2023 Sep 21:e2306808. doi: 10.1002/adma.202306808. PMID: 37732588
PubMed 37732588 DOI
Rizalar FS, Lucht MT, Petzoldt A, Kong S, Sun J, Vines JH, Telugu NS, Diecke S, Kaas T, Bullmann T, Schmied C, Löwe D, King JS, Cho W, Hallermann S, Puchkov D, Sigrist SJ, Haucke V. Phosphatidylinositol 3,5-bisphosphate facilitates axonal vesicle transport and presynapse assembly. Science. 2023 Oct 13;382(6667):223-230. doi: 10.1126/science.adg1075. Epub 2023 Oct 12.PMID: 37824668
PubMed 37824668 DOI
Singaram, I., Sharma, A., Pant, S., Lihan, M., Park, M.-J., Pergande, M., Buwaneka, P., Hu, Y. Mahmud, N. Kim, Y.-M. Cologna, S. Gevorgyan, V. Khan, I. Tajkhorshid, E. and Cho, W. Targeting lipid-protein interaction to treat Syk-mediated acute myeloid leukemia (2023) Nature Chemical Biology, 19, 230-250 DOI: 10.1038/s41589-022-01150-z/NCHEMB-A210913789 (Highlighted in Nature Reviews in Drug Discovery and Cancer Discovery)
PubMed 36229686 DOI

2022

Ha Pham, Indira Singaram, Jiachen Sun, Arthur Ralko, Madalyn Puckett, Ashutosh Sharma, Alice Vrielink, and Wonhwa Cho, Development of a novel spatiotemporal depletion system for cellular cholesterol (2022) J Lipid Res, 63, 100178. doi: 10.1016/j.jlr.2022.100178
PubMed 35143844 DOI
Liqian Ma, Wonhwa Cho, and Erik R. Nelson Our evolving understanding of how 27-hydroxycholesterol influences Cancer, (2022) Biochemical Pharmacology, 196:114621. doi: 10.1016/j.bcp.2021.114621
PubMed 34043965 DOI
Wonhwa Cho, Arthur Ralko, Ashutosh Sharma, and Shu-Lin Liu An In situ fluorescence assay for cholesterol transport-like activity of the Patched. (2022) Methods in Molecular Biology 2374, 37-47.
PubMed 34562241 DOI

2021

Buwaneka, P., Ralko, A., Gorai, S., Pham, H. & Cho, W. Phosphoinositide-binding activity of Smad2 is essential for its function in TGF-β signaling. Journal of Biological Chemistry 297
PubMed 34655614 DOI
Buwaneka, P., Ralko, A., Liu, S.-L. & Cho, W. Evaluation of the available cholesterol concentration in the inner leaflet of the plasma membrane of mammalian cells. J Lipid Res 62, 100084
PubMed 33964305 DOI
Ma, L., Cho, W. & Nelson, E. R. Our evolving understanding of how 27-hydroxycholesterol influences cancer. Biochem Pharmacol 114621
Yang, Fan, et al. The Impact of TiO2 Nanoparticle Exposure on Transmembrane Cholesterol Transport and Enhanced Bacterial Infectivity in HeLa Cells. Acta Biomaterialia., vol. 135, Elsevier, 2021, pp. 606–16
PubMed 34400307 DOI

2020

Koponen, A. et al. ORP2, a cholesterol transporter, regulates angiogenic signaling in endothelial cells. FASEB J 34, 14671–14694 (2020).
PubMed 32914503 DOI
Sharma, A. et al. Photostable and Orthogonal Solvatochromic Fluorophores for Simultaneous In Situ Quantification of Multiple Cellular Signaling Molecules. ACS Chem. Biol. 15, 1913–1920 (2020).
PubMed 32525312 DOI
Sun, J., Singaram, I., Soflaee, M. H. & Cho, W. A direct fluorometric activity assay for lipid kinases and phosphatases. Journal of Lipid Research 61, 945–952 (2020).
PubMed 32341006 DOI
Zhang, Y. et al. Hedgehog pathway activation through nanobody-mediated conformational blockade of the Patched sterol conduit. Proc Natl Acad Sci U S A 117, 28838–28846 (2020).
PubMed 33139559 DOI

2019

Bae, D.-J. et al. ArhGAP12 plays dual roles in Stabilin-2 mediated efferocytosis: Regulates Rac1 basal activity and spatiotemporally turns off the Rac1 to orchestrate phagosome maturation. Biochimica et Biophysica Acta (BBA) – Molecular Cell Research 1866, 1595–1607 (2019).
PubMed 31301364 DOI

2018

Kim E, Kim DH, Singaram I, Jeong H, Koh A, Lee J, Cho W*, and Ryu SH*. Cellular phosphatase activity of C1-Ten/Tensin2 is controlled by Phosphatidylinositol-3,4,5-triphosphate binding through the C1-Ten/Tensin2 SH2 domain (2018) Cell Signal. 51, 130-138 (*Co-corresponding authors)
PubMed 30092354 DOI
Liu, S.-L., Wang, Z., Hu, Y., Xin, Y., Gorai, S., Sharma, A., Zhou, X., Gong, L-W., Hay, N., Zhang, J. and Cho, W. Quantitative lipid imaging reveals a new signaling function of phosphatidylinositol-3,4-bisphosphate: Isoform and subcellular site-specific activation of Akt (2018) Molecular Cell 71, 1092-1104 doi: 10.1016/j.molcel.2018.07.035
PubMed 30174291 DOI
Zhang, Y., Bulkley, D.P., Xin, Y., Roberts, K.J., Asarnow, D.E., Sharma, A., Myers, B.R., Cho, W.*, Cheng, Y.*, Beachy*, P.A. Structural basis for cholesterol transport-like activity of the Hedgehog receptor Patched (2018) Cell 175, 1352-1364 (*Co-corresponding authors)
PubMed 30415841 DOI

2017

Ahn, G., Kim, H., Kim, D., Hanh, H., Yoon, Y., Zhuang, X., Liang, Z., Adu-Gyamfi, E., Stahelin, R.V., Jiang, L., Cho, W., Kang, B.-H., Hwang, I. (2017) SH3P2 as a key factor in membrane structure modulation during cell plate formation in plants Plant Cell 29, 1388-1405
Ahn, G., Kim, H., Kim, D.H., Hanh, H., Yoon, Y., Singaram, I., Wijesinghe, K.J., Johnson, K.A., Zhuang, X., Liang, Z., Stahelin, R.V., Jiang, L., Cho, W., Kang, B.H., Hwang, I. (2017) “SH3 Domain-Containing Protein 2 Plays a Crucial Role at the Step of Membrane Tubulation during Cell Plate Formation” Plant Cell 29, 1388-1405.
PubMed 28584166 DOI
Cho, W., Hu, Y., Baek, K., Kim, H. (2017) A High-throughput fluorometric assay for lipid–protein binding Methods Enzymol. 583, 1-18
Cho, W., Yoon, Y., Liu, S.-L., Baek, K., Sheng, R. (2017) Fluorescence-based in situ quantitative imaging for cellular lipids Methods Enzymol. 583, 19-33
Liu, S.-L., Sheng, R., Jung, J.H., Wang, L., Stec, E., O’Connor, M.J. Song, S., Bikkavilli, R.K., Baek, K., Winn, R.A., Lee, D., Ueda, K., Levitan, I., Kim, K.-P., and Cho, W. (2017) Orthogonal lipid sensors identify transbilayer asymmetry of plasma membrane cholesterol Nature Chemical Biology, 13, 268-274
PubMed 28024150 DOI
Wood, M. N., Ishiyama, N., Singaram, I., Chung, C., Flozak, A.S., Yemelyanov, A., Ikura, M., Cho, W. and Gottardi, C.J. (2017) α-Catenin homodimers are recruited to phosphoinositide-activated membranes to promote adhesion, J. Cell Biol. 216, 3767-3783.
PubMed 28874417 DOI

2016

Cho, W. Kim, H., Hu, Y. (2016) High-throughput fluorometric assay for membrane-protein interaction Methods in Mol. Biol. 1376, 163-174
PubMed 26552683 DOI
Francis, K.R., Ton, A.N., Xin, Y., O’Halloran, P.E., Wassif, C.A., Malik, N., Williams, I.M., Cluzeau, C.V., Trivedi, N.S., Pavan, W.J., Cho, W., Westphal, H., Porter, F.D. (2016) Modeling the cholesterol synthesis disorder Smith-Lemli-Opitz Syndrome with iPS cells reveals Wnt/b-catenin defects are causative for neural phenotype. Nature Medicine 22, 388-396 [Featured in News & Views]
PubMed 26998835 DOI
Komura, N., Suzuki, K. G. N., Ando, H., Konishi, M., Koikeda, M., Imamura, A., Chadda, R., Fujiwara, T.K., Tsuboi, H., Sheng, R., Cho, W., Ishida, H., Kusumi, A., Kiso, M. (2016) Raft-based interactions of gangliosides with a GPI-anchored receptor. Nature Chem. Biol. 12, 402-410
PubMed 27043189 DOI
Park, M-J, Sheng, R., Silkov, A., Jung, D-J, Wang, Z-G, Xin, Y., Kim, H., Thiagarajan-Rosenkranz, P., Song, S., Yoon, Y., Nam, W., Kim, I., Kim, E., Lee, D-G, Chen, Y., Singaram, I., Wang. L. Jang, M.H., Hwang, C.-S., Honig, B., Ryu, S.H., Lorieau, J., Kim, Y-M, and Cho, W. (2016) SH2 domains serve as lipid binding modules for pTyr-signaling proteins Mol. Cell 62, 7-20. [Highlighted in Nature Reviews in Molecular Cell Biology (May, 2016) and Cancer Discovery; Recommended in Faculty of 1000 Prime being of special significance (Prywes R: F1000Prime Recommendation of Evaluation [Park MJ et al., Mol Cell 2016, 62(1):7-20]. In F1000Prime, 14 Apr 2016; DOI: 10.3410/f.726271123.793516567. F1000Prime.com/726271123#eval793516567)]
PubMed 27052731 DOI
Sheng, R., Jung, D-J, Silkov, A., Kim, H., Singaram, I., Wang, Z-G, Xin, Y., Kim, E., Park, M-J, Rosenkranz, P.T., Smrt, S., Honig, B., Baek, K., Ryu, S.H., Lorieau, J., Kim, Y-M, and Cho, W. (2016) Lipids regulate Lck activity through their interactions with the Lck SH2 domain. J. Biol. Chem.291, 17639-50
PubMed 27334919 DOI

2015

Agarwal, S., Kim, H., Chen, R.B., Agarwal, S., Williamson, R., Cho, W., Di Paolo, G., and Satchell, K. J. F. (2015) Autophagy and endosomal trafficking inhibition by Vibrio cholerae MARTX toxin phosphatidylinositol-3-phosphate specific phospholipase A1 activity Nature Commun 6:8745. doi: 10.1038/ncomms9745.
PubMed 26498860 DOI
Tyson, G.H., Halavaty, A., Kim, H., Geissler, B., Agard, M., Cho, W., Anderson, W., Hauser, A. (2015) A Novel Phosphatidylinositol 4,5-bisphosphate Binding Domain Mediates Plasma Membrane Localization of ExoU and Other Patatin-like Phospholipases J. Biol. Chem. 290, 2919-2937
PubMed 25505182 DOI

2014

Bhardwaj, N., Cho, W., and Lu, H. (2014) MetaDoR: Online resource and prediction server for membrane targeting peripheral proteins in Algorithmic and Artificial Intelligence Methods for Protein Bioinformatics, Eds., Pan, Y., Li, M., Wang, J. Wiley Series on Bioinformatics, Wiley.
Kim, D.H., Park, M.-J., Gwon, G.H., Silkov, A., B. Xu, Z.-Y, Yang, E.C., Song, K., Song, K., Kim, Y., Yoon, H.S., Honig, B., Cho, W., Cho, Y., and Hwang, I.* (2014) An Ankyrin Repeat Domain of AKR2 Drives Chloroplast Targeting through Coincident Binding of Two Chloroplast Lipids Dev. Cell 30, 598-609 (*Co-corresponding authors)
PubMed 25203210 DOI
Kurilova, S., Lee, D., and Cho, W. (2014) Simultaneous In Situ Quantification of Two Cellular Lipid Pools Using Orthogonal Fluorescent Sensors Angew. Chem. Int. Ed. 53, 14387-14391
PubMed 25345859 DOI
Liu, S.-L., Sheng, R., O’Connor, M.J., Cui, Y., Yoon, Y.
Sheng, R., Kim, H., Lee, H., Xin, Y., Chen, Y., Tian, W., Choi, J.-C., Doh, J., Han, J.-K. and Cho, W. (2014) Cholesterol Selectively Activates Canonical Wnt Signaling over Non-Canonical Wnt Signaling. Nature Commun. 5:4393 doi: 10.1038/ncomms5393
PubMed 25024088 DOI

2013

Jung, H.-R., Cho, W., Choi, J.-C., Doh, J. (2013) Microfabricated platforms to modulate and monitor T cell synapse assembly, WIREs Nanomedicine & Nanobiotechnology
PubMed 22927231 DOI
Kim, H., Afsari, H. S., and Cho, W. (2013) High-Throughput Fluorescence Assay for Membrane-Protein Interaction J. Lip. Res. 54, 3531-3538
PubMed 24006510 DOI
Tsukamoto, K., Wales, T. E., Daniels, K., Pal, R., Sheng, R., Cho, W., Stafford, W., Engen, J. R., Krieger, M., and Kocher, O. (2013) Non-Canonical Role of the PDZ4 domain of the Adaptor Protein PDZK1 in the Regulation of the Hepatic HDL receptor SR-BI. J. Biol. Chem. 288,19845-19860
Yao, L.H., Rao, Y., Bang, C., Kurilova, S., Varga, K., Wang, C.Y., Weller, B.D., Cho, W., Cheng, J., Gong, L.W. (2013) “Actin polymerization does not provide direct mechanical forces for vesicle fission during clathrin-mediated endocytosis” J. Neurosci. 33, 15793-8.
PubMed 24089486 DOI

2012

BL, Levine AJ, Materi AM, Song WL, Keku TO, Goodman JE, Sansbury LB, Das S, Dai A, Kwong AM, Lin AM, Lin JM, Park JM, Patterson RE, Chlebowski RT, Garavito RM, Inoue T, Cho W, Lawson JA, Kapoor S, Kolonel LN, Le Marchand L, Haile RW, Sandler RS, Lin HJ. (2012) Hematopoietic prostaglandin D synthase (HPGDS): A high stability, Val187Ile isoenzyme common among African Americans and its relationship to risk for colorectal cancer. Prostaglandins Other Lipid Mediat. 97, 22-28. PMCID: PMC3226866
PubMed 21821144 DOI
Chen, Y., Sheng, R., Kallberg, M., Silkov, A., Tun, M.P., Bhardwaj, N., Kurilova, S., Hall, R.A., Honig, B., Lu, H., and Cho, W. (2012) Genome-Wide Functional Annotation of Dual Specificity Protein- and Lipid-Binding Modules That Regulate Protein Interactions Molecular Cell 46, 226-237.PMCID: PMC3431187 [Featured in Faculty of 1000, ScienceDaily (Cell Protein Interactions Favor Fats) and UIC News (Fats are control freaks that want to rule the cells)
PubMed 22445486 DOI
Gil, J.-E., Kim, E., Kim, I.-S., Ku, B., Park, W.S., B.-H. Oh, Ryu, S. H., Cho, W., Heo W.D (2012) Phosphoinositides differentially regulate protrudin localization through the FYVE domain (*corresponding authors) J. Biol. Chem. 287, 41268-41276 PMID: 23043110
PubMed 23043110 DOI
K. Song, M. Jang, S. Y. Kim, Y. J. Lee, W. Cho, and I. Hwang (2012). An A/ENTH domain-containing protein co-localizes with clathrin-coated vesicles on the growing cell plate in Arabidopsis root cells Plant Physiol 159,1013-25
PubMed 22635117 DOI
M. Rowland, D. Gong, H. E. Bostic, N. Lucas, W. Cho, and M. D. Best (2012) Microarray Analysis of Akt PH Domain Binding Employing Synthetic Biotinylated Analogs of All Seven Phosphoinositide Headgroup Isomers Chem. Phys. Lipids 165, 207-215. doi: 10.1016/j.chemphyslip.2011.12.001.
PubMed 22178158 DOI
Sheng, R., Chen, Y., Gee, H. Y., Stec, E., Melowic, H.R., Blatner, N.R., Tun, M.P, Kim, Y., Kallberg, M., Fujiwara, T., Hong, J.H., Kim, K.P., Lu, H., Kusumi, A., Lee, M.G.and Cho W. (2012) Cholesterol Modulates Cell Signaling and Protein Networking by Specifically Interacting with PDZ Domain-Containing Scaffolding Proteins Nature Communications 3, 1249 DOI: 10.1038/ncomms2221 [Featured in National Institute of General Medical Sciences (NIH NIGMS) News and UIC News
PubMed 23212378 DOI
Stahelin, R.V., Kong, K-F., Raha, S., Tian, W., Melowic, H., Murray, D., Altman, A., and Cho W., (2012) Protein kinase Cθ C2 domain is a phosphotyrosine-binding module that plays a key role in its activation. J. Biol. Chem. 287, 30518-30528 PMCID: PMC3436300
PubMed 22787157 DOI
Yoon, Y., Zhang, X., Cho, W. (2012) “Phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) specifically induces membrane penetration and deformation by Bin/amphiphysin/Rvs (BAR) domains” J. Biol. Chem. 287, 34078-90.
PubMed 22888025 DOI

2011

Chen, H., Málková, Š., Cho, W, and Schlossman, M.L. (2011) Configuration of membrane-bound proteins by x-ray reflectivity J. Appl. Phys. 110, 102215
J. K. Bolger, W. Tian, W. R. Wolter, W. Cho, M. A. Suckow and M. J. Miller (2011) Synthesis and evaluation of 5-lipoxygenase translocation inhibitors from acylnitroso hetero-Diels Alder cycloadducts Org Biomol Chem. 9, 2999-3010
PubMed 21365098 DOI
Long, F. Cho, w., and Ishii, Y. (2011) Expression and purification of 15N- and 13C-isotope labeled 40-residue human Alzheimer’s peptide for NMR-based structural analysis, Prot. Purif. 9,16-24 PMID: 21640828
PubMed 21640828 DOI
Lucas, N. and Cho, W., (2011) Phosphatidylserine Binding Is Essential for Plasma Membrane Recruitment and Signaling Function of PDK1 J. Biol. Chem. 286, 41265-72
PubMed 21971045 DOI
McCary, CA, Yoon, Y., Panagabko, C, Cho, W., Atkinson, J. and Cook-Mills, J., (2011) Vitamin E isoforms directly bind PKC and differentially regulate activation of PKC Biochem. J. 441,189-198
PubMed 21933153 DOI
Morales KA, Lasagna M, AV, Yoon Y, Reinhart GD, Lee JC, Cho W, Li P, Igumenova TI (2011) Pb>2+ as modulator of protein-membrane interactions J Am Chem Soc.133,10599-10611 PMID: 21615172
PubMed 21615172 DOI
Rowland, M., Bostic, H., Gong, D., Spears, A., Lucas, N., Cho, W., Cravatt, B., and Best, M. (2011) Phosphatidylinositol (3,4,5)-trisphosphate activity probes for the labeling and proteomic characterization of protein binding partners Biochemistry. 50, 11143-61. PMID: 22074223
PubMed 22074223 DOI
Silkov, A., Yoon, Y., Lee, H., Gokhale, N., Stahelin, R.V., and Cho, W* and Murray, D.* (2011) Genome-Wide Identification of Lipid Binding Domains and Prediction of Their Membrane Binding Properties: An ANTH/ENTH domain Study J. Biol. Chem 286, 34155-34163 (*co-corresponding authors)
Yoon, Y., Lee, P.J., Kurilova, S., and Cho, W. (2011), In Situ Quantitative Imaging of Cellular Lipids Using Molecular Sensors Nature Chemistry 3, 868-74. PMID: 22024883 [Featured in ASBMB Today (New protein sensors to quantify phosphoinositides in situ), RSC Chemistry World (New probe throws light on cellular lipids), ScienceDaily (New Membrane Lipid Measuring Technique May Help Fight Disease) and UIC News]
PubMed 22024883 DOI

2010

Capraro, B. R., Yoon, Y., Cho, W., and Baumgart T. (2010), Curvature sensing by the epsin N-terminal homology (ENTH) domain measured on cylindrical lipid membrane tethers J. Amer. Chem. Soc. 132,1200-1 PMID: 20050657
PubMed 20050657 DOI
Yoon, Y., Tong, J. Lee, P.J., Albanese, A., Bhardwaj, N., Digman, M.A., Lu, H., Gratton, E., Shin, Y.-K. and Cho, W., (2010) Molecular basis of the potent membrane remodeling activity of the epsin1 ENTH domain J. Biol. Chem., 285, 531-540
PubMed 19880963 DOI

2009

Chen W, Goldfine H, Ananthanarayanan B, Cho W, Roberts MF. (2009) Listeria monocytogenes Phosphatidylinositol-Specific Phospholipase C: Kinetic Activation and Homing in on Different Interfaces (dagger), Biochemistry, 48, 3578-92.
PubMed 19281241 DOI
Chen, C.-H., Malkova, S., Long, F., Garde, S., Cho, W., and Schlossman, M. L.. (2009) Configuration of PKCa-C2 Domain Bound to SOPC:SOPS Lipid Monolayers, Biophys. J. 97, 2794 – 2802
PubMed 19917234 DOI
Denghuang Gong, Matthew D. Smith, Debasis Manna, Heidi Bostic, Wonhwa Cho and Michael D. Best (2009) Microplate-Based Characterization of Protein-Phosphoinositide Binding Interactions Using Synthetic Biotinylated Head Group Analogues, Bioconj. Chem 20, 310-6.
PubMed 19182890 DOI
Lee, J.S., Kim, I.S., Kim, J.H., Cho, W., Suh, P.G., Ryu, S.H. (2009) Determination of EGFR endocytosis kinetic by auto-regulatory association of PLD1 with mu2. PLoS One. 4(9):e7090
PubMed 19763255 DOI
Meerschaert, K., Tun, M.P., Remue, E., De Ganck, A., Boucherie, C., Vanloo, B., Degeest, G., Vandekerckhove, J., Zimmermann, P., Bhardwaj, N., Lu, H., Cho, W., Gettemans, J. (2009) “The PDZ2 domain of zonula occludens-1 and -2 is a phosphoinositide binding domain” Cell. Mol. Life Sci. 66, 3951-66.
PubMed 19784548 DOI
Meerscharet, K., Tun, M.P., Remue, E., De Ganck, A., Houthuys, E., Boucherie, C., Vanloo, B., Degeest, G., Vandekerckhove, J., Zimmermann, P., Bhardwaj, N., Lu, H., Cho, W., Gettemans, J. (2009), The PDZ domain of Zona Occuldens-1 and -2 is a phosphoinositide-binding domain. Cell. Mol. Life Sci.. 66:3951–3966 (*co-corresponding authors).

2008

Das A, Base C, Manna D, Cho W, Dubreuil RR. (2008) “Unexpected complexity in the mechanisms that target assembly of the spectrin cytoskeleton,” J. Biol. Chem. 283, 12643-2653 [PMID: 18287096]
PubMed 18287096 DOI
Manna, D., Bhardwaj, N., Vora, M.S., Stahelin, R.V., Lu, H., and Cho, W., (2008) Relative Importance of Phosphatidylserine and PtdIns(4,5)P2 in Plasma Membrane Targeting of Cellular Proteins: Molecular Dynamics Simulation and Membrane Binding Studies of the PKCα C2 Domain J. Biol. Chem. 283, 26047–26058.
PubMed 18621733 DOI
Tian, W., Wijewickrama, G. T., Kim, J. H., Das, S., Tun, M. P., Gokhale, N., Jung, J.W., Kim, K. P., and Cho, W. “Mechanism of Regulation of Group IVA Phospholipase A2 Activity by Ser727 Phosphorylation,” J. Biol. Chem. 283, 3960-3971
PubMed 18065419 DOI

2007

Bhardwaj, N., Stahelin, R.V., Cho, W., Lu, H. (2007) “MeTaDoR: A comprehensive resource for membrane targeting domains and their host proteins,” Bioinformatics 23, 3110-3112
PubMed 17720983 DOI
Blatner, N.R., Wilson, M.I. Lei, C., Hong, W., Murray, D., Williams, R.L. and Cho, W. (2007) “The Structural Basis of Novel Endosome Anchoring Activity of KIF16B Kinesin,” EMBO J. 26, 3709-3719
PubMed 17641687 DOI
Chaudhari, A., Mahfouz, M., Fialho, A.M., Yamada, T., Granja, A.T., Zhu, Y., Hashimoto, W., Schlarb-Ridley, B., Cho, W., Gupta, T.D., and Chakrabarty, A.M. (2007) “Cupredoxin – Cancer Interrelationship: Azurin Binding with EphB2, Interference in EphB2 Tyrosine Phosphorylation, and Inhibition of Cancer Growth” Biochemistry 46, 1799-1810
PubMed 17249693 DOI
Hom, R., Vora, M., Regner, M., Subach, O.M., Cho, W., Verkhusha, V.V., Stahelin R.V., and Kutateladze, T.G. (2007) “pH-dependent binding of the EHTH domain of epsin and the ANTH domain of AP180 to PI(4,5)P2-containing bilayers” J. Mol. Biol. 373, 412-23
PubMed 17825837 DOI
Kwatia, M.A, Doyle, C.B, Cho, W., Enhorning, G., and Ackerman, S.J (2007) “The Combined Activities of Secretory Phospholipases and Eosinophil Lysophospholipases Induce Pulmonary Surfactant Dysfunction by Phospholipid Hydrolysis,” J. Allergy Clin Immunol 119, 838-847.
Lamour, N.F., Stahelin, R.V., Wijesinghe, D.S., Maceyka, M., Wang, E., Allegood, J.C., Cho, W., Merrill, Jr., A.H., and Chalfant, C.E. (2007) “Ceramide kinase utilizes ceramide provided by ceramide transport protein. Localization to subcellular compartments of eicosanoid synthesis” J. Lipid Res. 48, 1293-304
PubMed 17392267 DOI
Manna, D. and Cho, W. (2007) “Real-Time Cell Assays of Phospholipases A2 Using Fluorogenic Phospholipids,” Methods Enzymol. 434, 15-27
PubMed 17954240 DOI
Manna, D., Albanese, A., Park, W.S., and Cho, W. (2007) “Mechanistic Basis of Differential Cellular Responses of PtdIns(3,4)P2- and PtdIns(3,4,5)P3-Binding PH Domains,” J. Biol. Chem. 282, 32093-32105
Melowic, H.R., Stahelin, R.V., Blatner, N.R., Tian, W., Hayashi, K., Altman, A., and Cho, W., (2007) “Mechanism of Diacylglycerol-Induced Membrane Targeting and Activation of Protein Kinase Cθ” J. Biol. Chem. 282, 21467-76
PubMed 15105418 DOI
Muñoz, N.M., Meliton, A.Y., Arm, J.P., Zhu, X., Bonventre, J.V., Cho, W., and Leff. A.R. (2007) “Deletion of Secretory Group V Phospholipase A2 Attenuates Cell Migration and Airway Hyperresponsiveness in Immune-Sensitized Mice,” J. Immunol. 179, 4800-4807
PubMed 17878379 DOI
Stahelin, R.V., Karathanassis, D.,Murray, D., Williams, R.L., Cho, W. (2007) “Structural and Membrane Binding Analysis of the PX Domain of Bem1p: Basis of Phosphatidylinositol-4-Phosphate Specificity” J. Biol. Chem. 282, 25737-25747
PubMed 17581820 DOI
Stahelin, R.V., Subramanian, P., Vora, M., Cho, W., and Chalfant. C.E., (2007) “Ceramide-1-phosphate binds group IVA cytosolic phospholipase A2 via a novel site in the C2 domain,” J. Biol. Chem. 282, 20467-74
PubMed 17472963 DOI

2006

Bhardwaj, N., Stahelin, R.V., Langlois, R.E., *Cho, W., and *Lu, H. (2006), “Structural Bioinformatics Prediction of Membrane-Binding Proteins” J. Mol Biol. 359, 486-495 (* Co-corresponding authors)
PubMed 16626739 DOI
Chaudhari, A., Fialho, A.M., Ratner, D., Gupta, P., Hong, C.S., Kahali, S., Yamada, T., Haldar, K., Cho, W., Chauhan, V.S., Gupta, T.D., and Chakrabarty, A.M. (2006) “Azurin, Plasmodium falciparum Malaria and HIV/AIDS: Inhibition of Parasitic and Viral Growth by Azurin,” Cell Cycle 5:15, 1642-1648
PubMed 16861897 DOI
Cho, W. (2006) “Building Signaling Complexes at the Membrane” Science STKE 2006, pe7 (2006)
PubMed 16467194 DOI
Cho, W. (2006) “Real-Time Fluorescent Assays of Phospholipases” ACS Chem Biol, 1, 65-66
Cho, W. (2006) “Seeing is believing: real-time cellular activity assay for phospholipase A2” ACS Chem. Biol. 1, 65-6.
PubMed 17163643 DOI
Cho, W. (2006) “Special Issue: Lipid-binding domains,” Biochim. Biophys. Acta (Preface) 1761, 803-804.
Cho, W. and Stahelin, R. V. (2006) “Membrane binding and subcellular targeting of C2 domains,” Biochim. Biophys. Acta (Review) 1761, 838-849.
PubMed 16945584 DOI
Malkova, S., Stahelin, R.V., Pingali, S.V., Cho, W., and Schlossman, M.L. (2006) “X-ray Reflectivity Studies of p40phox-PX Domains Adsorbed onto Langmuir Monolayers of SOPC/SOPS/PI3P” Biochemistry 45, 13566-13575
PubMed 15994899 DOI
Mallikaratchy, P. Stahelin, R.V., Zehui Cao, Z., Cho, W., and Tan, W., (2006) “Selection of DNA ligands for protein kinase C-δ” Chem. Comm, 2006, 3229-3231
PubMed 17028752 DOI
Muñoz, N.M. Meliton, A.Y. Lambertino, A., Boetticher, E., Learoyd, J., Sultan, F., Zhu, X., Cho, W., and Leff, A.R. (2006) “Transcellular secretion of group V phospholipase A2 from epithelium induces β-integrin mediated adhesion and synthesis of leukotriene C4 in eosinophil,” J. Immunol 177, 574-82
PubMed 16785555 DOI
Málková, S., Stahelin, R.V., Pingali, S.V., Cho, W., Schlossman, M.L. (2006) “Orientation and penetration depth of monolayer-bound p40phox-PX” Biochemistry 45, 13566-75.
PubMed 17087510 DOI
S. Lee, J. Kovacs, R. V. Stahelin, M. L. Cheever, C. Burd, W. Cho and T. G. Kutateladze (2006) “Molecular mechanism of membrane docking by the Vam7p PX domain,” J. Biol. Chem. 281, 37091-37101
PubMed 16984909 DOI
Stahelin, R.V., Karathanassis, D. Bruzik, K.S., Waterfield, M.D., Bravo, J., Williams, R.L., Cho, W., (2006) “Structural and Membrane Binding Analysis of the PX Domain of Phosphoinositide 3-Kinase-C2α,” J. Biol. Chem. 281, 39396-39406.
PubMed 17038310 DOI
Wijewickrama, G., Kim, J.-H., Kim, Y.J., Ananthanarayanan, B., Abraham, A. Oh, Y. Kwatia, M., Ackerman, S. and Cho, W. (2006) “Systematic Evaluation of Transcellular Activities of Secretory Phospholipases A2: HIGH ACTIVITY OF GROUP V PLA2 TO INDUCE EICOSANOID BIOSYNTHESIS IN NEIGHBORING INFLAMMATORY CELLS,” J. Biol. Chem. 281, 10935-10944
PubMed 16476735 DOI
Wijewickrama, G.T. Albanese, A., Kim, Y. J., Oh, Y.S., Murray, P.S., Takayanagi, R., Tobe, T., Masuda, S., Murakami, M., Kudo, I., Ucker, D.S., Murray, W., Cho, W., (2006) “Unique Membrane Interaction Mode of Group IIF Phospholipase A2” J. Biol. Chem. 281, 32741-32754
PubMed 16931517 DOI

2005

Angulo, Y, Gutiérrez, J.M., Soares, A.M., Cho, W., and Lomonte, B. (2005) “Myotoxic and cytolytic activities of dimeric Lys49 phospholipase A2 homologues are reduced, but not abolished, by a pH-induced dissociation,” Toxicon, 46(3), 291-6
PubMed 15970303 DOI
Cho, W. and Stahelin, R.V. “In vitro and cellular membrane binding mechanisms of membrane targeting domains,” (2005) In “Protein-Lipid Interactions”, Tamm, L. K. Ed., Academic Press, N.Y. pp. 369-401
PubMed 11432875 DOI
Cho, W. and Stahelin, R.V. “Membrane-protein interactions in cell signaling and membrane trafficking,” (2005) Ann. Rev. Biophys. Biomol. Struct. 34, 119-151
PubMed 15869386 DOI
Gokhale, N. A. Abraham, A. Digman, M.A., Gratton, E., and Cho, W. (2005) “Phosphoinositide specificity of and mechanism of lipid domain formation by annexin A2-p11 tetramer” J. Biol. Chem. 280, 42831-42840
PubMed 16230353 DOI
Malkova, S., Long, F., Stahelin, R.V. Pingali, S.V., Murray, D., Cho, W., and Schlossman, M. (2005) “Determination of the depth of membrane insertion of C2 domains by X-ray reflectivity measurements” Biophys. J. 89, 1861-1873
Stahelin, R.V. Digman, M.A., Medkova, M., Ananthanarayanan, B., Rafter, J.D., Melowic, H. and Cho, W. (2005) “Diacylglycerol-Induced Membrane Targeting and Activation of Protein Kinase Cε: MECHANISTIC DIFFERENCES BETWEEN PKCδ and ε” J. Biol. Chem. 280, 19784-19793
PubMed 17548359 DOI
Stahelin, R.V., Hwang, J.H., Kim, J.-H., Park, Z.-Y., Johnson, K.R., Obeid, L.M., and Cho, W. (2005) “The mechanism of membrane targeting of human sphingosine kinase 1,” J. Biol. Chem. 280, 43030-43038
PubMed 16243846 DOI
Stahelin, R.V., Wang, J., Blatner, N.R. Rafter, J.D., Murray, D., and Cho, W. (2005) “The origin of C1A-C2 interdomain interactions in protein kinase Cα” J. Biol. Chem. 280, 36452-36463
PubMed 16079140 DOI
Subramanian, P., Stahelin, R.V., Szulc, Z., Bielawska, A., Cho, W. and Chalfant, C.E. (2005) “Ceramide-1-phosphate acts as a positive allosteric activator of cytosolic phospholipase A2 and enhances the interaction of the enzyme with phosphatidylcholine” J. Biol. Chem. 280, 17601-17607
PubMed 15743759 DOI
Taha, T.A., Kitatani, K., Bielawski, J., Cho, W., Hannun, Y. A., and Obeid, L.M. (2005) “TNF induces the loss of sphingosine kinase-1 by a cathepsin B dependent mechanism,” J. Biol. Chem. 280, 17196-17202
PubMed 15710602 DOI

2004

Blatner, N.R., Stahelin, R.V., Diraviyam, K. Bruzik, K.S., Hawkins, P.T., Hong, W., Murray, D., and Cho, W. (2004) “The Molecular Basis of the Differential Subcellular Localization of FYVE Domains,” J. Biol. Chem. 279, 53818-53827
PubMed 15452113 DOI
Stahelin, R.V. Digman, M.A., Medkova, M. Ananthanarayanan, B., Rafter, J.D., Melowic, H. and Cho, W. (2004) “Mechanisms of diacylglycerol-induced membrane binding and activation of protein kinase Cd” J. Biol. Chem. 279, 29501-29512.
Stahelin, R.V., Ananthanarayanan, B., Blatner, N.R., Singh, S., Bruzik, K.S. Murray, D., and Cho, W. (2004) “Mechanism of Membrane Binding of the Phospholipase D1 PX Domain,” J. Biol. Chem. 279, 54918-54926.
PubMed 15475361 DOI

2003

Ananthanarayanan, B., Stahelin, R. V., Digman, M. A., and Cho, W. (2003) “Activation Mechanisms of Protein Kinase C Isoforms Are Determined by the Ligand Affinity and Conformational Flexibility of Their C1 Domains,” J. Biol. Chem. 278, 46886-46894.
PubMed 12954613 DOI
Cho, W., Digman, M.A., Ananthanarayanan, B. and Stahelin, R.V. (2003) “Bacterial expression and purification of C1 and C2 domains of protein kinase C isoforms” in Methods in Molecular Biology, vol. 233: Protein Kinase C Protocols, Ed., Newton, A.C., K. Humana Press, Totowa, New Jersey, 291-298.
PubMed 12840516 DOI
Das, S., Dixon, J.E. and Cho, W. (2003) “Mechanism of membrane targeting and activation of PTEN,” Proc. Natl. Acad. Sci. USA 100, 7491-7496.
PubMed 12808147 DOI
Das, S., Rafter, J.D., Kim, K.-P., Gigy, S.P., and Cho, W. (2003) “Mechanism of group IVA cytosolic phospholipase A2 activation by phosphorylation,” J. Biol. Chem. 278, 41431-41442.
PubMed 12885780 DOI
Diraviyam, K., Stahelin, R. V., Cho, W., and Murray, D. (2003) “Computer modeling of the membrane interaction of FYVE domains,” J. Mol. Biol. 328, 721-736
PubMed 12706728 DOI
Lee, B.-I., Mustafi, D., Cho, W., and Nakagawa, Y. (2003) “Characterization of Calcium Binding Properties of Lithostathine,” J. Biol. Inorg. Chem. 8, 341-347
PubMed 12589570 DOI
Liu, Y., Bruzik, K.S., Ananthanarayanan, B., and Cho, W. (2003) “New aspects of formation of 1,2-cyclic phosphate by phospholipase C-d1,” Bioorg. Med. Chem. Lett. 11, 2471-2475
PubMed 12735994 DOI
Murakami, M, Das, S., Kim, Y.J., Cho, W., Kudo, I. (2003), “Perinuclear localization of cytosolic phospholipase A2α is crucial but notobligatory for coupling with cyclooxygenases” FEBS Lett 546, 251-256
PubMed 12832050 DOI
Muñoz, N.M., Kim, Y.J., Kim, K.-P., Zhu, X., Leff, A.R., and Cho, W (2003) “Group V Phospholipase A2 Induces Group IVA Phospholipase A2-Independent Cysteinyl Leukotriene Biosynthesis in Human Eosinophils,” J. Biol. Chem. 278, 38813-38820
PubMed 12796497 DOI
Ryu, Y., Oh, Y., Yoon, J., Cho, W., Baek, K. (2003) “Molecular characterization of a gene encoding the Drosophila melanogaster phospholipase A2” Biochim. Biophys. Acta 1628, 206-10.
PubMed 12932833 DOI
Stahelin, R.V. Burian, A., Bruzik, K.S., Murray, D. and Cho, W. (2003) “Membrane binding mechanisms of the PX domains of NADPH oxidase p40phox and p47phox,” J. Biol. Chem. 278, 14469-14479
PubMed 12556460 DOI
Stahelin, R.V. Forslund, R.E., Wink, D.J. and Cho, W. (2003) “Development of a biochemistry laboratory course with a project-oriented goal” Biochemistry and Molecular Biology Education. 31, 106-112
Stahelin, R.V., Long, F., Murray, D. Peter, B.J., De Camilli, P., McMahon, H.T., and Cho, W. (2003) “Contrasting membrane binding mechanisms of ENTH and ANTH domains,” J. Biol. Chem. 278, 28993-28999
Stahelin, R.V., Rafter, J.D., Das, S. and Cho, W. (2003) “A Molecular Basis of Differential Subcellular Localization of C2 Domains of Protein Kinase C-α and Group IVa Cytosolic Phospholipase A2,” J. Biol. Chem. 278, 12452-12460
PubMed 12531893 DOI
Wehbi, H., Feng, J., Kolbeck, J., Ananthanarayanan, B., Cho, W., and Roberts, M. F. (2003) “Investigating the Interfacial Binding of Bacterial Phosphatidylinositol-Specific Phospholipase C” Biochemistry 42, 9374-9382
PubMed 12899624 DOI

2002

Ananthanarayanan, B., Das, S., Rhee, S.G., Murray, D. and Cho, W. (2002) “ Membrane Targeting of C2 Domains of Phospholipase C-d Isoforms” J. Biol. Chem. 277, 3568-3575.
PubMed 11706040 DOI
Beers, S.A., Buckland, A.G., Koduri, R.S. Cho, W., Gelb, M.H., and Wilton, D.C. (2002) “The antibacterial properties of secreted phospholipase A2: A major physiological role for the IIA enzyme that depends on the very high pI of the enzyme to allow penetration of the bacterial cell wall” J. Biol. Chem. 277, 1788-1793
PubMed 11706041 DOI
Das, S. and Cho, W. (2002) “Roles of Catalytic Domain Residues in Interfacial Binding and Activation of Group IV Cytosolic Phospholipase A2,” J. Biol. Chem. 277, 23838–23846.
PubMed 11956215 DOI
Gesquire, L., Cho, W., and Subbaiah, P.V. (2002) “Roles of group IIa and group V secretory phospholipases A2 in the metabolism of lipoproteins. Substrate specificities of the enzymes, and the regulation of their activities by sphingomyelin,” Biochemistry 41, 4911-4920.
PubMed 11939786 DOI
Han, J.M., Kim, J.H., Lee, B.D., Lee, S.D., Kim, Y., Chung, Y.W., Lee, S., Cho, W., Ohba, M., Kuroki, T., Suh, P.-G. and Ryu, S.H. (2002) “Phosphorylation-dependent regulation of Phospholipase D2 by Protein Kinase C in Rat pheochromocytoma PC12 cells,” J. Biol. Chem. 277, 8290-8297.
PubMed 11744693 DOI
Karathanassis, D., Stahelin, R.V., Bravo, J., Perisic, O., Pacold, C. M., Cho, W., Williams, R.L. (2002) “Binding of the PX domain of p47phox to phosphatidylinositol 3,4-bisphosphate and phosphatidic acid is masked by an intramolecular interaction” EMBO J. 21, 5057-5068
PubMed 12356722 DOI
Kim, Y.J., Kim, K.P., Han, S.K., Munoz, N.M., Zhu, X., Sano, H, Leff, A.R., and Cho, W. (2002) “Group V Phospholipase A2 Induces Leukotriene Biosynthesis in Human Neutrophils through the Activation of Group IVA Phospholipase A2″ J. Biol. Chem. 277, 36479-36488
PubMed 12124392 DOI
Kim, Y.J., Kim, K.P., Rhee, H. J., Das, S. Rafter, J.D., Oh, Y.S., and Cho, W. (2002) “Internalized Secretory Phospholipase A2 Acts on the Perinuclear Membranes” J. Biol. Chem. 277, 9358-65.
PubMed 11777916 DOI
Kulkarni, S., Das, S., Funk, C.D., Murray, D., and Cho, W. (2002) “A molecular basis of specific subcellular localization of the C2-like domain of 5-lipoxygenase” J. Biol. Chem. 277, 13167-13174.
PubMed 11796736 DOI
Muñoz, N.M., Boetticher, E., Sperling, A.I., Kim, K.P., Meliton, A., Lambertino, A., Cho, W., and Leff, A.R. (2002) “Deveolpment of highly sensitive sandwich enzyme-linked immunosorbent assay (sELISA) for group V phospholipase A2 (gVPLA2): determination of gVPLA2 in human epithelial cells and eosinophils” J. Immunol. Methods, 262, 41-51.
PubMed 11983218 DOI
Stahelin, R. V. Long, F., Diraviyam, K., Bruzik, K.S., Murray, D. and Cho, W. (2002) “Phosphatidylinositol-3-Phosphate Induces the Membrane Penetration of the FYVE Domains of Vps27p and Hrs” J. Biol. Chem. 277, 26379-26388.
PubMed 12006563 DOI

2001

Bittova, L., Stahelin, R. V. and Cho, W. (2001) “Roles of Ionic Residues of the C1 Domain in Protein Kinase C-α Activation and the Origin of Phosphatidylserine Specificity,” J. Biol. Chem. 276, 4218-4226.
PubMed 11029472 DOI
Cho, W. (2001) “Membrane targeting by C1 and C2 domains,” J. Biol. Chem. (minireview) 276, 32407-32410.
Cho, W., Bittova, L., and Stahelin, R. V. (2001) “In vitro Membrane Binding Assays for Peripheral Proteins” Anal. Biochem. (review article) 296, 153-161.
PubMed 11554709 DOI
Kim, K.-P., Rafter, J. D., Bittova, L., Han, S.-K., Snitko, Y., Muñoz, N.M., Leff, A. R., and Cho, W (2001) “Mechanism of Human Group V Phospholipase A2-Induced Leukotriene Biosynthesis in Human Neutrophils: A Potential Role of Heparan Sulfate Binding in PLA2 Internalization and Degradation,” J. Biol. Chem. 276, 11126–11134.
PubMed 11118430 DOI
KomStahelin, R. V. and Cho, W. (2001) “Roles of ionic, aliphatic, and aromatic residues in membrane-protein interactions: A surface plasmon resonance study on phospholipases A2,” Biochemistry 40, 4672-4678
PubMed 11294634 DOI
Myou, S., Sano, H., Fujimura, M., Zhu, X., Kurashima, K., Kita, T., Nakao, S., Nonomura, A., Takanobu, S., Kim, K.P., Muñoz, N. M., Cho, W., and Leff, A. R. (2001) “Inhibition of cytosolic PLA2 and lysophospholipid synthesis blocks antigen-induced eosinophil migration and airway hyper responsiveness,” Nature Immunol 2, 145-149
Stahelin, R. V. and Cho, W. (2001) “Roles of Calcium Ions in Membrane Binding and Subcellular Targeting of C2 Domains” Biochem. J. 359, 679-685
PubMed 11672443 DOI
Zhu, X., Sano, H., Kim, K.P., Sano, A., Boetticher, E., Muñoz, N.M., Cho, W., Leff, A.R. (2001) “Role of mitogen-activated protein kinase-mediated cytosolic phospholipase A2 activation in arachidonic acid metabolism in human eosinophils” J. Immunol. 167, 461-8.
PubMed 11418683 DOI
Zhu, X., Sano, H., Kim, K.P., Sano, A., Boetticher, E., Muñoz, N.M., Cho, W., and Leff, A.R. (2001) “Role of MAP Kinase-Mediated Cytosolic Phospholipase A2 Activation in Arachidonic Acid Metabolism in Human Eosinophils” J. Immunol 167, 461-468
PubMed 11418683 DOI

2000

Bitto, E., Li, M., Tikhonov, A., Schlossman, M. L., Cho, W. (2000) “Mechanism of vesicle aggregation by annexin I,” Biochemistry 39, 13469-13477
PubMed 11063583 DOI
Cho, W. (2000) “Structure and function of group V phospholipase A2,” Biochim. Biophys. Acta (review article) 1488, 48-58.
PubMed 11080676 DOI
Kim, K., Han, S.-K., Hong, M., and Cho, W (2000) “Molecular Basis for Phosphatidylcholine Preference of Human Group V Phospholipase A2,” Biochem. J. 348, 643-647
PubMed 10839997
Muñoz, N. M., Kim, K., Han, S.-K., Boetticher, E. Sperling, A. I. Sano, H. Zhu, X. Cho, W., and Leff, A. R. (2000) “Characterization of Monoclonal Antibodies Specific for 14 kDa human group V Secretory Phospholipase A2,” Hybridoma 19, 171-176

1999

Bitto, E. and Cho, W. (1999) “A Structural Determinant of the Vesicle Aggregation Activity of Annexin I,” Biochemistry 38, 14094-14100
PubMed 10529257 DOI
Bittova, L., Sumandea, P.M. and Cho, W. (1999) “A Structure-Function Study of C2 Domain of Cytosolic Phospholipase A2: Identification of essential calcium ligands and hydrophobic membrane binding residues” J. Biol. Chem. 274, 9665-9672
PubMed 10092653 DOI
Cho, W. and Shen, Z. (1999) “Efficient Immobilization of Phospholipase A2” in Methods in Molecular Biology, vol. 109: Lipase and Phospholipase Protocols, Eds, Doolittle, M.H. and Reue, K. Humana Press, Totowa, New Jersey pp. 303-307
PubMed 9918031 DOI
Cho, W., Han, S.-K., Lee, B.-I., Snitko, Y. and Dua, R. (1999) “Purification and Assay of Mammalian Group I and Group IIa Secretory Phospholipase A2” in Methods in Molecular Biology, vol. 109: Lipase and Phospholipase Protocols, Eds, Doolittle, M.H. and Reue, K. Humana Press, Totowa, New Jersey pp 31-38
PubMed 9918009 DOI
Cho, W., Wu, S.-K., Yoon, E.T. and Lichtenbergova, L. (1999) “Fluorometric Phospholipase Assays Based on Polymerized Liposome Substrates” in Methods in Molecular Biology, vol. 109: Lipase and Phospholipase Protocols, Eds, Doolittle, M.H. and Reue, K. Humana Press, Totowa, New Jersey pp.7-17
PubMed 9918007 DOI
Gelb, M.H., Cho, W. and Wilton, D.C. (1999) “Interfacial Binding of Secreted Phospholipases A2: More Than Electrostatics and a Major Role for Tryptophan” Current Opinions in Structural Biology 9, 428-432
Han, S.-K., Kim, K.P., Koduri, R., Bittova, L., Munoz, N.M., Leff, A. R., Wilton, D.C., Gelb, M.H., and Cho, W. (1999) “Role of Trp31 in high membrane binding and pro-inflammatory activity of human group V phospholipase A2” J. Biol. Chem. 274, 11881-11888
PubMed 10207008 DOI
Lee, B.-I., Dua, R. and Cho, W. (1999) “A Structural Determinant of the Unique Interfacial Binding Mode of Bovine Pancreatic Phospholipase A2” Biochemistry 38, 7811-7818
PubMed 10387021 DOI
Lomonte, B. Angulo, Y. Rufini, S. Cho, W. Giglio, J.R. Ohno, M. Daniele, J.J, Geoghegan, P. and Gutiérrez, J.M. (1999) “Comparative study of the cytolytic activity of myotoxic phospholipases A2 on mouse endothelial (tEnd) and skeletal muscle (C2C12) cells in vitro” Toxicon 37(1):145-58
Medkova, M. and Cho, W. (1999) “Interplay of the C1 and C2 Domains of Protein Kinase C-α in Its Membrane Binding and Activation” J. Biol. Chem 274, 19852-19861
PubMed 10391930 DOI
Snitko, Y., Han, S.-K. and Cho, W. (1999) “Interfacial and Substrate Binding of Human Pancreatic Phospholipase A2: A Comparison among Mammalian Pancreatic Phospholipases A2” Biochemistry 38, 7803-7810
PubMed 10387020 DOI
Sumandea, M., Das, S., Sumandea, C. and Cho, W. (1999) “Roles of Aromatic Residues in High Interfacial Activity of Naja naja atra Phospholipase A2,” Biochemistry 38, 16290-16297
PubMed 10587453 DOI
Wilson, H.A., Waldrip, J.B., Nielson, K.H., Judd, A.M., Han, S.-K., Cho, W., Sims, P.J., and Bell, J.D. (1999) “Mechanisms by which elevated intracellular calcium induces S49 cell membranes to become susceptible to the action of secretory phospholipase A2” J. Biol. Chem. 274, 11494-11504
Zhu, X., Muñoz, N.M., Kim, K.-P., Sano, H., Cho, W, and Leff, A. R., (1999) “Cytosolic Phospholipase A2 Activation is Essential for β1 and β2 Integrin-Dependent Adhesion to Human Eosinophils,” J. Immunol 163, 3423-3429
PubMed 10477614

1998

Bitto, E. and Cho, W. (1998) “Roles of Individual Domains of Annexin I in Its Vesicle Binding and Vesicle Aggregation: A Comprehensive Mutagenesis Study” Biochemistry 37, 10231-10237
PubMed 9665730 DOI
Ghosh, A. K., K. Krishnan, D. E. Walters, W. Cho, Y. Koo, H. Cho, L. Holland, J. Buthod (1998) “Structure Based Design: Novel Spirocyclic Ethers as Nonpeptidal P2-Ligands for HIV Protease Inhibitors” Bioorg. Med. Chem. Lett. 8, 979-982
PubMed 9871524 DOI
Ghosh, A.K., Kinkaid, J.F., Cho, W., Walters, D.E., Krishnan, K., Hussain, K.A., Koo, Y., Cho, H., Rudall, C., Holland, L., Buthod (1998) “Potent HIV Protease Inhibitors Incorporating High Affinity P2-Ligands and (R)-(Hydroxyethylamino)-Sulfonamide Isostere” Bioorg. Med. Chem. Lett. 8, 687-690
PubMed 9871583 DOI
Han, S.-K., Yoon, E.T. and Cho, W. (1998) “Functional Expression and Characterization of Human Secretory Class V Phospholipase A2” Biochem. J. (Communication) 331, 353-357
PubMed 9531469 DOI
Koduri, R., Baker, S.F., Snitko, Y., Han, S.-K., Cho, W., Wilton, D. C. and Gelb, M. H. (1998) “Action of Human Group IIa Secreted Phospholipase A2 on Cell Membranes: Vesicle but not Heparanoid Binding Determines Rate of Fatty Acid Release” J. Biol. Chem. 273, 32142-32153
Lichtenbergova, L., Yoon, E. T. and Cho, W. (1998) “Membrane Penetration of Cytosolic Phospholipase A2 is Necessary for Its Interfacial Catalysis and Arachidonate Specificity” Biochemistry 37, 14128-14136
PubMed 9760249 DOI
Lin, Y. Ghomashchi, F., Nielsen, R., Snitko, Y., Yu, B.-Z., Han, S.-K., Cho, W., Wilton, D. C., Jain, M.K., Robinson, B.H. and Gelb, M. H. (1998) “Binding of bee venom and human group IIa phospholipases A2 to membranes: a minor role for electrostatics.” Biochem Soc Trans. 26, 341-5
Medkova, M. and Cho, W. (1998) “Differential membrane-binding and activation mechanisms of Protein Kinase C-α and -ε” Biochemistry 37, 4892-4900
PubMed 9538007 DOI
Medkova, M. and Cho, W. (1998) “Mutagenesis of the C2 Domain of Protein Kinase C-α: Differential Roles of Ca2+ Ligands and Membrane Binding Residues” J. Biol. Chem. 273, 17544-17552
PubMed 9651347 DOI

1997

Han, S.-K., Lee, B.-I., and Cho, W. (1997) “The Bacterial Expression and Kinetic Characterization of Human Pancreatic Phospholipase A2” Biochim. Biophys. Acta 1346, 185-192
PubMed 9219901 DOI
Han, S.-K., Yoon, E. T., Scott, D.L., Sigler, P.B. and Cho, W. (1997) “Structural Aspects of Interfacial Adsorption: A Crystallographic and Site-directed Mutagenesis Study of the Phospholipase A2 from the Venom of Agkistrodon piscivorus piscivorus” J. Biol. Chem. 272, 3573-3582
PubMed 9013608
Kim, Y., Lichtenbergova, L., Snitko, Y., and Cho, W. (1997) “A Phospholipase A2 Kinetic and Binding Assay Using Phospholipid-Coated Hydrophobic Beads” Anal. Biochem. 250, 109-116
PubMed 9234904 DOI
Snitko, Y., Koduri, R., Han, S.-K., Othman, R., Baker, S.F., Molini, B. J., Wilton, D. C., Gelb, M. H., and Cho, W. (1997) “Mapping the Interfacial Binding Surface of Human Secretory Class II Phospholipase A2” Biochemistry 36, 14325-14333
PubMed 9398150 DOI
Snitko, Y., Yoon, E. T. and Cho, W. (1997) “High Specificity of Human Secretory Class II Phospholipase A2 for Phosphatidic Acid” Biochem. J. 321, 737-741
PubMed 9032461 DOI

1996

Lee, B.-I., Yoon, E.T. and Cho, W. (1996) “Roles of Surface Hydrophobic Residues in the Interfacial Binding of Bovine Pancreatic Phospholipase A2” Biochemistry 35, 4231-4240
PubMed 8672459 DOI
Mukhopadhyay, S. and Cho, W. (1996) “Interactions of Annexin V with Phospholipid Monolayers“ Biochim. Biophys. Acta 1276, 58-62
PubMed 8624362 DOI

1995

Dua, R., Wu, S.-K., and Cho, W. (1995) “A Structure-Function Study of Bovine Pancreatic Phospholipase A2 Using Polymerized Mixed Liposomes” J. Biol. Chem. 270, 263-268
PubMed 7814384 DOI
Shen, Z. and Cho, W. (1995) “Highly Efficient Site-Specific Immobilization of Phospholipases A2” J. Lipid Res. 36, 1147-1151
Shen, Z. and Cho, W. (1995) “Membrane Leakage Induced by Synergetic Action of Lys-49 and Asp-49 Agkistrodon Piscivorus Piscivorus Phospholipases A2: Implications in Their Pharmacological Activities” Int. J. Biochem. Cell Biol. 27/10, 1009-1013
Shen, Z., Cho, W. (1995) “Highly efficient immobilization of phospholipase A2 and its biomedical applications” J. Lipid Res. 36, 1147-51.
PubMed 7658163

1994

Dua, R. and Cho, W. (1994) “Inhibition of Human Secretory Class II Phospholipase A2 by Heparin” Eur. J. Biochem. 221, 481-490
PubMed 8168536 DOI
Shen, Z., Wu, S.-K. and Cho, W. (1994) “Effects of Specific Fatty-Acid Acylation of Phospholipase A2 on Its Interfacial Binding and Catalysis” Biochemistry 33, 11598-11607
PubMed 7918373 DOI
Wu, S.-K. and Cho, W. (1994) “A continuous Fluorometric Assay for Phospholipases Using Polymerized Mixed Liposomes” Anal. Biochem. 221, 152-159
PubMed 7985786 DOI

1993

Wu, S.-K. and Cho, W. (1993) “Use of Polymerized Liposomes to Study Interactions of Phospholipase A2 with Biological Membranes” Biochemistry 32, 13902-13908
PubMed 8268165 DOI

1991

Cho, W. and Kézdy, F. J. (1991) “Chromogenic Phospholipase A2 Substrates and Assays” Methods Enzymol. 197, 75-78

1988

Cho, W., Markowitz, M.A., and Kézdy, F.J. (1988) “A New Class of Phospholipase A2 Substrates: Kinetics of the Phospholipase A2-Catalyzed Hydrolysis of 3-Acyloxy-4-Nitrobenzoic Acids” J. Am. Chem. Soc. 110, 5166-5171
Cho, W., Tomasseli, A.G., Heinrikson, R.L., and Kézdy, F.J. (1988) “The Chemical Basis for Interfacial Activation of Monomeric Phospholipase A2” J. Biol. Chem. 263, 11237-11241

1985

Suh, J., Cho, W., and Chung, S. (1985) “Carboxypeptidase A-Catalyzed Hydrolysis of α-(Acylamino)-cinnamoyl Derivatives of L-β-Phenyllactate and L-Phenylalanate: Evidence for Acyl-Enzyme Intermediates” J. Am. Chem. Soc. 107, 4530-4535

1984

Maraganore, J.M., Merutka, G., Cho, W., Welches, W., Kézdy, F.J., and Heinrikson, R.L. (1984) “A New Class of Phospholipase A2 with Lysine in Place of Aspartate 49: Functional Consequences of Calcium and Substrate Binding” J. Biol. Chem. 259, 13839-13843